Three strains of virus designated CPDV-92A, CPDV-92B and CPDV-93A were isolated from the contagious pustular dermatitis(CPD)lesions of the Korean native goat. The isolates caused cytopathic effects in primary goat testicle cell and vero cell. In
direct
fluorescent assay using anti CPDV conjugate, positive reactions were specifically detected in the cytoplasm of the infected cells. All isolates were antigenically related to each other and the reference CPDV strains by cross-neutralization test.
In the physicochemical and biological properties. the isolates were not affected by BUDR treat ment suggesting virus with DNA genome. All isolates were resistant to lipid solvents such as ether and chloroform, and labile at pH 3.0 and 8.5, and
heat
treatment. In millipore filteration, the infectivity was significantly decreased at 220nm in pore size. In electron microscopical examination, the ovoid particles measured 140-260nm in size were observed with a crisscross pattern of surface
filaments.
In pathogenicity, no clinical signs was manifested in mouse and guinea pig, wherease Korean native goats showed the typical skin lesions following intradermal injection of the virus.
The hemorrhagic pocks were present on the chorioallantoic membrane of the embryonated eggs. In SDS-PAGE analysis of structural protein, five major and eight minor bands were detected showing no difference in the band pattern among the isolates.
In
Western immunoblotting analysis, the sera obtained from the immunized goats reacted specifically with 45K and 37K. The inactivated antigen and tissue culture-attenuated CPDV induced the various degree of humoral and cell-mediated immune responses
in the
goat. The former generated lower immune response than the latter.
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